The NEXTFLEX® Unique Dual Index Barcodes are barcoded adapters, for sequencing on Illumina® platforms, that provide unprecedented data security in sequencing applications. Multiplexing with NEXTfFLEX® Unique Dual Index Barcodes drastically increases scale while reducing costs by allowing the user to pool multiple libraries in a single flow cell lane. These barcoded adapters can be used with single read or paired-end read sequencing, and are compatible with NEXTFLEX® library prep kits designed for Illumina® sequencing, or other workflows for Illumina® sequencing that involve the ligation of adapters to adenylated fragments.
Each NEXTFLEX® Unique Dual Index barcodes (1-1,536) contains 1,536 unique dual-index barcodes, enabling users to multiplex up to 1,536 libraries per flow cell lane. Each lot of the NEXTFLEX® Unique Dual Index barcodes (1-1,536) is functionally validated and tested for index purity by sequencing. NEXTFLEX® Unique Dual Index barcodes are supplied in 96-well plates with each well containing 2 reactions of each barcode. These kits ship on dry ice. The shelf life of each reagent is at least two years when stored properly.
Learn more about the NEXTflex® Unique Dual Index Barcodes.