The NEXTFLEX® Rapid XP DNA-Seq kit combines enzymatic fragmentation with end-repair and A-tailing in one reaction to create a highly efficient first step in library generation, which is followed by ligation and PCR (optional for PCR-free workflows). This effective one-tube workflow produces DNA-seq libraries with consistent library size, high yield, low GC-bias, and high coverage.
The NEXTFLEX® Rapid DNA-Seq kit contains enough library prep reagents and cleanup beads to prepare 8 or 48 DNA samples for Illumina® sequencing. The shelf life of all reagents is at least 6 months when stored properly. The Nuclease-free Water and Resuspension Buffer can be stored at room temperature. The NEXTFLEX® Cleanup Beads XP should be stored at 4°C, and all other components should be stored at -20°C. The NEXTFLEX® Cleanup Beads XP ships at room temperature, while other components ship on dry ice. Learn more about the NEXTFLEX® Rapid XP DNA-Seq kit.